Genetic diversity and amplification of different clostridial [FeFe] hydrogenases by group-specific degenerate primers

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چکیده

منابع مشابه

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Among the large variety of micro-organisms capable of fermentative hydrogen production, strict anaerobes such as members of the genus Clostridium are the most widely studied. They can produce hydrogen by a reversible reduction of protons accumulated during fermentation to dihydrogen, a reaction which is catalysed by hydrogenases. Sequenced genomes provide completely new insights into the divers...

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MAD-DPD: designing highly degenerate primers with maximum amplification specificity.

This work introduces minimum accumulative degeneracy, a variant of the degenerate primer design problem, which is particularly useful when a large number of sequences are to be covered by a set of restricted number of primers. A primer set, which is designed on a minimum accumulative degeneracy basis, especially helps to reduce nonspecific PCR amplification of undesired DNA fragments, as fewer ...

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ژورنال

عنوان ژورنال: Letters in Applied Microbiology

سال: 2011

ISSN: 0266-8254

DOI: 10.1111/j.1472-765x.2011.03135.x